Viral Bcl2s' transmembrane domain interact with host Bcl2 proteins to control cellular apoptosis

Viral control of programmed cell death relies in part on the expression of viral analogs of the B-cell lymphoma 2 (Bcl2) protein known as viral Bcl2s (vBcl2s). vBcl2s control apoptosis by interacting with host pro- and anti-apoptotic members of the Bcl2 family. Here, we show that the carboxyl-terminal hydrophobic region of herpesviral and poxviral vBcl2s can operate as transmembrane domains (TMDs) and participate in their homo-oligomerization. Additionally, we show that the viral TMDs mediate interactions with cellular pro- and anti-apoptotic Bcl2 TMDs within the membrane. Furthermore, these intra-membrane interactions among viral and cellular proteins are necessary to control cell death upon an apoptotic stimulus. Therefore, their inhibition represents a new potential therapy against viral infections, which are characterized by short- and long-term deregulation of programmed cell death

La creación de un nuevo mercado alimentario en España: las vitaminas en la prensa periódica (1917-1950) The creation of a new nutritional market in Spain: vitamins in the press (1917-1950)

Objetivo: Analizar el grado en el que las vitaminas jugaron un papel en la estrategia de creación de un nuevo mercado de alimentos, alimentos-medicamento y especialidades farmacéuticas en España durante la primera mitad del siglo XX. Material y métodos: Se estudiaron todas las menciones a las vitaminas aparecidas en los diarios "ABC", "Heraldo de Madrid", "El Imparcial", "El Liberal" y "El Sol", publicados en Madrid; "La Vanguardia", publicado en Barcelona, y en el semanario "Blanco y Negro" entre 1917 y 1950. La fecha inicial, 1917, se fijó por ser el año en el que aparecieron las primeras noticias sobre vitaminas. La fecha final, 1950, se fijó por considerarse concluida la etapa más dura de la autarquía impuesta por el Franquismo. Las noticias aparecidas se clasificaron en las categorías: consejos dietéticos, curiosidades, noticias científicas, noticias económicas, noticias políticas, trivialidades y uso metafórico. Resultados: Los anuncios que usaron las vitaminas dentro de su estrategia comercial son el apartado más importante en el que se utiliza el concepto vitaminas con porcentajes superiores, en casi todos los casos, al 50%. Las noticias científicas sobre vitaminas le siguen en importancia. Las menciones a las vitaminas en el resto de categorías son inferiores al 5%. Conclusiones: Las vitaminas constituyen un elemento fundamental en la creación de un nuevo mercado de productos en España en la primera mitad del siglo XX, a juzgar por las menciones que aparecen en la prensa periódica analizada.Aim: To analyze the extent to which vitamins played a role in the strategy to create a new market of food, food-medicines, and medicines in Spain during the first half of the twentieth century. Materials and methods: A study was made of references to vitamins in the years between 1917 and 1950 in the daily newspapers "ABC", "Heraldo de Madrid", "El Imparcial", "El Liberal" and "El Sol", published in Madrid, "La Vanguardia", published in Barcelona, and the weekly publication "Blanco y Negro". The starting date was selected as the year in which the first news of vitamins appeared. The year 1950 marked the end of the harshest stage of autarky imposed by Franco's regime. The news that appeared was classified in the following categories: scientific news, nutritional advice, advertising, economy, politics, anecdotes and metaphors. Results: The advertisements that used vitamins as part of their marketing strategy are the most important section with over 50% of total references to the vitamin concept in almost all the newspapers. Scientific news on vitamins ranks second in importance. References to vitamins in the remaining categories were below 5%. Conclusions: Judging by the references which appear in the most important newspapers of the time vitamins represent a fundamental element in the creation of a new food product market in Spain in the first half of the twentieth century

Drought response in forest trees : from the species to the gene

Forest tree species, considering their long lifespan, symbolize one of the best biological examples of adaptation to a frequently changing harsh terrestrial environment. The adaptation to environments with water scarcity was the first challenge in the evolution of terrestrial photosynthetic organisms, and prompted the development of strategies and mechanisms to cope with drought. In this respect, the particular evolution and life history of forest tree species have brought about a plethora of specific adaptations to dry environments. The presence of a hydraulic system for long distance water transport and the need of maintaining functional tissues and organs for long periods of time are two important characteristics making forest tree species singular organisms within the plant kingdom. Selective pressure has prompted a variety of strategies in the control of water losses to maintain the functionality of the hydraulic system without compromising the carbon balance of the plant. These and other physiological responses focussed to increase the dehydration tolerance of tissues (e.g., osmotic adjustment) have played an important role in the development of specific adaptations under water limiting conditions. The adaptive changes are observable at different scales: from the population to the species, from the individual to the gene. The advance of high-throughput technologies will enable to unveil the complex interplay between phenotype and genotype. Genomic, proteomic, transcriptomic, and metabolomic approaches are beginning to bring light to the molecular basis of adaptation to drought in forest tree species. These new technologies, combined with more traditional approaches, will improve our current knowledge of the functional and molecular basis underlying adaptation and evolution of forest tree species living under dry environments. In this respect, this chapter covers some aspects of adaptation to drought at different integrative levels, from an ecophysiological perspective to a molecular-based point of view

3-Deazaadenosine alleviates senescence to promote cellular fitness and cell therapy efficiency in mice

Cellular senescence is a stable type of cell cycle arrest triggered by different stresses. As such, senescence drives age-related diseases and curbs cellular replicative potential. Here, we show that 3-deazaadenosine (3DA), an S-adenosyl homocysteinase inhibitor, alleviates replicative and oncogene-induced senescence. 3DA-treated senescent cells showed reduced global histone H3 lysine 36 trimethylation, an epigenetic modification that marks the bodies of actively transcribed genes. By integrating transcriptome and epigenome data, we demonstrate that 3DA treatment affects key factors of the senescence transcriptional program. Notably, 3DA treatment alleviated senescence and increased the proliferative and regenerative potential of muscle stem cells from very old mice in vitro and in vivo. Moreover, ex vivo 3DA treatment was sufficient to enhance the engraftment of human umbilical cord blood cells in immunocompromised mice. Together, our results identify 3DA as a promising drug enhancing the efficiency of cellular therapies by restraining senescence